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MOLECULAR DEFENSE SYSTEMS AGAINST OXIDATIVE STRESS AND PROTEOMICS - Grupo PAI: BIO-0216 |
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Current members of the team |
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| Doctors |
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e-mail |
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| José Antonio Bárcena Ruiz, Professor of Biochemistry and Molecular Biology (HEAD) |
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| Emilia Martínez Galisteo, Lecturer of Biochemistry and Molecular Biology |
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| Carmen Alicia Padilla Peña, Lecturer of Biochemistry and Molecular Biology |
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| José Peinado Peinado, Lecturer of Biochemistry and Molecular Biology |
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| Manuel José Rodríguez Ortega, Posdoctoral scientist, Ramón y Cajal fellow |
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| Brian McDonagh, Posdoctoral scientist |
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| Raúl González Ojeda, Contratado "Juan de la Cierva" |
[email protected] |
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| PhD students |
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| Alfonso Olaya Abril |
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| Irene Jiménez Munguía |
[email protected] |
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| Technicians |
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| Lourdes Laura Muñoz Gómez |
[email protected] |
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| Collaborators |
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| Miguel Carmona Cabello,Barchelor in Biochemistry |
[email protected] |
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| Barchelor students |
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| Pedro Miranda |
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| María José López Grueso |
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El grupo pertenece al Instituto Maimónides de Investigación Biomédica (IMIBIC) y al Campus de Excelencia Internacional Agroalimentario (ceiA3) |
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Group address |
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Edificio Severo Ochoa, 1ª planta, Campus de Rabanales. 14071 Córdoba. Spain
Teléfono: +34-957 21 85 90 / +34-957 21 86 86 / +34-957 21 83 17 |
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Research interests |
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| 1. The thiolic redox proteome of a cell is a set of proteins with particular cysteine residue(s) prone to oxidation by reactive oxygen and nitrogen species (ROS and RNS) in a reversible manner. Such a modification can serve two objectives: to protect the proteins from further lethal irreversible oxidation and to regulate the functionality of the affected proteins. The reversibility of the modification is dependent on the so called “redoxins”, a group proteins, among which Glutaredoxins interact with the universal reductant glutathione. |
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The research line supervised by Dra. Alicia Padilla studies the molecular and functional properties of redoxins in the yeast S. cerevisiae as a model organism with the construction of site directed mutants followed by biochemical, molecular and physiological analysis |
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Dr. McDonagh develops second generation and quantitative proteomics methods for the identification of proteins that make up the redox proteome together with a precise definition of the modified Cys residue(s) and quantitation of the modification extent. |
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Dr. González Ojeda extrapolates this experimental approach to cultures human cell lines affected in their nitric oxide (NO) metabolism and to immunosuppressed mice in the context of cell proliferation and tumor development. |
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2. The line under the supervision of Dr. Rodríguez Ortega applies a proteomics approach to study the surface proteins in pathogen microorganisms as candidates for the development of vaccines and diagnostic tools. The study extends to the human pathogens Streptococcus. pneumoniae and Staphilococcus aureus and the porcine epizootic pathogen Streptococcus suis. |
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3. Dr. Peinado is involved in the determination of the antioxidant capacity of natural products from local varieties of wines and grapevine. |
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Collaborations |
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- Profs. José Eugenio Sánchez Criado y Francisco Gaytán Luna, Grupo de Fisiología de la Reproducción, Facultad de Medicina, UCO
- Dr. José Rafael Pedrajas, Universidad de Jaén.
- Prof. Arne Holmgren, Karolinska Institute, Estocolomo, Suecia
- Prof. Giuseppe Teti, University of Messina, Italy.
- Prof. David Sheehan, University College Cork, Irland.
- Prof. J.Moreno, Depto. Química Agrícola, Universidad de Córdoba.
- Dr. J.Muntané, Instituto de Biomedicina de Sevilla (IBIS), Sevilla.
- Prof. Carmen Tarradas Luque, Departamento de Sanidad Animal, UCO.
- Prof. Julian de la Torre Cisneros, Hospital Universitario Reina Sofía, Córdoba.
- Dr. Ignacio Obando Santaella, Hospital Virgen de Valme, Sevilla.
- Prof. Jesús Díez Dapena, Departamento de Bioquímica y biología Molecular, UCO.
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Selected publications |
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Buffoni, L., R. Zafra, A. Perez-Ecija, F. J. Martinez-Moreno, E. Martinez-Galisteo, T. Moreno, J. Perez and A. Martinez-Moreno (2010). "Immune response of goats immunised with glutathione S-transferase and experimentally challenged with Fasciola hepatica." Parasitol Int 59(2): 147-153. doi: 10.1016/j.parint.2009.12.005 IF: 2.259
Peinado, J., N. López de Lerma and R. peinado (2010). "Synergistic antioxidant interaction between sugars and phenolics from a sweet wine." Eur Food Res Technol 231(3): 363-370. IF: 1.585
Sheehan, D., B. McDonagh and J. A. Bárcena (2010). "Redox proteomics." Expert review of proteomics 7(1): 1-4. doi:10.1586/epr.09.98 IF: 4.406
Zafra, R., R. Perez-Ecija, L. Buffoni, R. Mendes, A. Martinez-Moreno, F. Martinez-Moreno, E. M. Galisteo and J. Perez (2010). "Evaluation of hepatic damage and local immune response in goats immunized with native glutathione S-transferase of Fasciola hepatica." J Comp Pathol 143(2-3): 110-119. doi:10.1016/j.jcpa.2010.01.011 IF: 1.529
Hu, W., S. Tedesco, B. McDonagh, J. A. Bárcena, C. Keane and D. Sheehan (2010). "Selection of thiol- and disulfide-containing proteins of Escherichia coli on activated thiol-Sepharose." Analytical Biochemistry 398(2): 245-253. doi:10.1016/j.ab.2009.11.002 IF: 3.236
López de Lerma, N., J. Peinado, J. Moreno and R. A. Peinado (2010). "Antioxidant activity, browning and volatile Maillard compounds in Pedro Ximénez sweet wines under accelerated oxidative aging." LWT - Food Science and Technology 43(10): 1557-1563. IF: 2.292
Mcdonagh, B., R. Requejo, C. A. Fuentes-Almagro, S. Ogueta, J. A. Bárcena and C. A. Padilla (2011). "Thiol redox proteomics identifies differential targets of cytosolic and mitochondrial glutaredoxin-2 isoforms in Saccharomyces cerevisiae. Reversible S-glutathionylation of DHBP synthase (RIB3)." J Proteomics 74: 2487-2497. doi:10.1016/j.jprot.2011.04.018 I.F. 4.878
González, R., A. Cruz, G. Ferrín, P. López-Cillero, R. Fernández-Rodríguez, J. Briceño, M. A. Gómez, S. Rufián, M. D. l. Mata, A. Martínez-Ruiz, J. J. G. Marin and J. Muntané (2011). "Nitric oxide mimics transcriptional and post-translational regulation during α-tocopherol cytoprotection against glycochenodeoxycholate-induced cell death in hepatocytes." Journal of hepatology 55(1): 133-144. doi:10.1016/j.jhep.2010.10.022 I.F.: 9,334
González, R., G. Ferrín, P. Aguilar-Melero, I. Ranchal, C. I. Linares, R. I. Bello, M. de la Mata, V. Gogvadze, J. A. Bárcena, J. M. Alamo, S. Orrenius, F. J. Padillo, B. Zhivotovsky and J. Muntané (2012). "Targeting Hepatoma Using Nitric Oxide Donor Strategies." ANTIOXIDANTS & REDOX SIGNALING. doi:10.1089/ars.2011.4476 I.F. 8.456
McDonagh, B., M. A. Domínguez-Martín, G. Gómez-Baena, A. López-Lozano, J. Diez, J. A. Bárcena and J. M. García Fernández (2012). "Nitrogen starvation induces extensive changes in the redox proteome of Prochlorococcus sp. strain SS120." Environmental Microbiology Reports 4(2): 257-267. doi:10.1111/j.1758-2229.2012.00329.x I.F. 3.232
Olaya-Abril, A., L. Gómez-Gascón, I. Jiménez-Munguía, I. Obando and M. J. Rodríguez-Ortega (2012). "Another turn of the screw in shaving Gram-positive bacteria: Optimization of proteomics surface protein identification in Streptococcus pneumoniae." Journal of Proteomics 75(12): 3733-3746. doi:10.1016/j.jprot.2012.04.037 I.F. 4.878
Gómez-Gascón, L., I. Luque, A. Olaya-Abril, I. Jiménez-Munguía, R. A. Orbegozo-Medina, E. Peralbo, C. Tarradas and M. J. Rodríguez-Ortega (2012). "Exploring the pan-surfome of Streptococcus suis: Looking for common protein antigens." Journal of Proteomics 75(18): 5654-5666. doi:10.1016/j.jprot.2012.07.025 I.F. 4.878
McDonagh, B., P. Martínez-Acedo, J. Vázquez, C. A. Padilla, D. Sheehan and J. A. Bárcena (2012). "Application of iTRAQ Reagents to Relatively Quantify the Reversible Redox State of Cysteine Residues." International Journal of Proteomics 2012: 1-9. doi:10.1155/2012/514847 |
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